rabbit polyclonal anti cd163 antibody  (Boster Bio)

Journal: Frontiers in Oncology

Article Title: Tumor-associated macrophage expression in colorectal adenomas and carcinomas: relationship to Helicobacter pylori infection

doi: 10.3389/fonc.2025.1649619

Figure Lengend Snippet: Immunohistochemical detection of CD163 + and CD86 + TAMs in colorectal tissues (×200). (A) CD163 staining in various tissues. (B) CD86 staining. (C) IOD for CD163. (D) IOD for CD86. Data expressed as M (Q1, Q3) (n = 109). * P < 0.05, ** P < 0.01, *** P < 0.001 (DB-adjusted). Groups: 1, Normal; 2, CAS; 3, SSA; 4, CRC. M: Median; Q 1 : 1st Quartile; Q 3 : 3rd Quartile.

Article Snippet: The following reagents and equipment were used in this study: mouse monoclonal anti-CD163 antibody (10D6, ma5-11458, Invitrogen, Waltham, MA02451, USA); rabbit monoclonal anti-CD86 antibody (EP1158-37, ab269587, Abcam, Cambridge, UK); rabbit Polyclonal a nti-CD68 antibody ( GB113150 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD163 antibody ( GB113152 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD86 antibody ( GB115630 , Servicebio, Wuhan, China); secondary antibodies and DAB chromogenic kits (Biomiky, Biosharp, and Servicebio, respectively).

Techniques: Immunohistochemical staining, Staining

Journal: Frontiers in Oncology

Article Title: Tumor-associated macrophage expression in colorectal adenomas and carcinomas: relationship to Helicobacter pylori infection

doi: 10.3389/fonc.2025.1649619

Figure Lengend Snippet: Correlation of CD163 + /CD86 + TAMs levels with CRC development. (A) Differential expression between H.pylori -infected and uninfected groups. (B) Correlation between CD163 + and CD86 + expression. (C, D) Positive correlation of both markers with malignancy grade. Groups: 1, Normal; 2, CAS; 3, SSA; 4, CRC. *** P < 0.001 (MWU test).

Article Snippet: The following reagents and equipment were used in this study: mouse monoclonal anti-CD163 antibody (10D6, ma5-11458, Invitrogen, Waltham, MA02451, USA); rabbit monoclonal anti-CD86 antibody (EP1158-37, ab269587, Abcam, Cambridge, UK); rabbit Polyclonal a nti-CD68 antibody ( GB113150 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD163 antibody ( GB113152 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD86 antibody ( GB115630 , Servicebio, Wuhan, China); secondary antibodies and DAB chromogenic kits (Biomiky, Biosharp, and Servicebio, respectively).

Techniques: Quantitative Proteomics, Infection, Expressing

Journal: Frontiers in Oncology

Article Title: Tumor-associated macrophage expression in colorectal adenomas and carcinomas: relationship to Helicobacter pylori infection

doi: 10.3389/fonc.2025.1649619

Figure Lengend Snippet: Multiplex immunofluorescence (200×) : CD68 + (red), CD163 + (green), CD86 + (green), DAPI (nuclei, blue), Merge (multichannel overlay), Scale bar: 50 μm. (A) CD68 + CD163 + dual-positive cells (IF). (B) CD68 + CD86 + dual-positive cells (IF).

Article Snippet: The following reagents and equipment were used in this study: mouse monoclonal anti-CD163 antibody (10D6, ma5-11458, Invitrogen, Waltham, MA02451, USA); rabbit monoclonal anti-CD86 antibody (EP1158-37, ab269587, Abcam, Cambridge, UK); rabbit Polyclonal a nti-CD68 antibody ( GB113150 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD163 antibody ( GB113152 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD86 antibody ( GB115630 , Servicebio, Wuhan, China); secondary antibodies and DAB chromogenic kits (Biomiky, Biosharp, and Servicebio, respectively).

Techniques: Multiplex Assay, Immunofluorescence

Journal: Frontiers in Oncology

Article Title: Tumor-associated macrophage expression in colorectal adenomas and carcinomas: relationship to Helicobacter pylori infection

doi: 10.3389/fonc.2025.1649619

Figure Lengend Snippet: Changes in cell density and proportion of CD68 + CD163 +/ CD68 + CD86 + dual-positive TAMs across groups. Groups: 1, Normal; 2, CRA; 3, CRC. *** P < 0.001 (Tukey HSD).

Article Snippet: The following reagents and equipment were used in this study: mouse monoclonal anti-CD163 antibody (10D6, ma5-11458, Invitrogen, Waltham, MA02451, USA); rabbit monoclonal anti-CD86 antibody (EP1158-37, ab269587, Abcam, Cambridge, UK); rabbit Polyclonal a nti-CD68 antibody ( GB113150 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD163 antibody ( GB113152 , Servicebio, Wuhan, China); rabbit Polyclonal anti-CD86 antibody ( GB115630 , Servicebio, Wuhan, China); secondary antibodies and DAB chromogenic kits (Biomiky, Biosharp, and Servicebio, respectively).

Techniques:

Journal: Acta biomaterialia

Article Title: A subtype specific probe for targeted magnetic resonance imaging of M2 tumor-associated macrophages in brain tumors.

doi: 10.1016/j.actbio.2025.01.003

Figure Lengend Snippet: Fig. 6. Immunohistochemical and FACS validations of M2pep-uIONP targeting M2 TAMs. Confocal microscopic images of tumor tissues collected from mice at 24 h after i.v. injection of probes show (A) Cy7-M2pep-uIONP were highly co-localizing with CD68- and CD163-double positive M2 TAM, revealing the targeting specificity. In comparison, the non-targeted control probe Cy7-scM2pep-uIONP and Cy7-Ferumoxytol not only exhibited much less tumoral accumulation, but also were not co-localized with M2 TAM. Scale bars: 37 µm. (B) Flow cytometric zebra plots and (C) the corresponding quantification of CD68- and CD163-double positive M2 TAM targeted by FITC-M2pep-uIONP, FITC-scM2pep-uIONP, and FITC-Ferumoxytol. The gating strategy involves sorting CD163+ M2 TAM population in live cells dissociated from mouse cerebrums by gating on CD68+ pan-macrophage populations, followed by further gating to assess the FITC-labeled nanoparticles.

Article Snippet: Rabbit polyclonal anti-CD163 antibody was purchased from Boster Biological Technology (Pleasanton, CA, USA).

Techniques: Immunohistochemical staining, Injection, Comparison, Control, Labeling

Journal: iScience

Article Title: T cell death-associated gene 8-mediated distinct signaling pathways modulate the early and late phases of neuropathic pain

doi: 10.1016/j.isci.2024.110955

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-CD163 , Biorbyt , Cat#: ORB13303; NA.

Techniques: Binding Assay, Recombinant, Software